Exact(4)
The catalysts were very efficient in the phenol oxidation reaction in diluted aqueous medium under mild experimental conditions (298 K and atmospheric pressure).
The catalysts were very efficient in the phenol oxidation reaction in diluted aqueous medium under mild experimental conditions (298 K and atmospheric pressure) and in the CO oxidation reaction.
Subsequently the amino group of compound 1 was converted to N-t-Boc derivative by reaction with (Boc 2O in aqueous medium under mild conditions.
A resuspended P6 pellet derived from 60 ml conditioned medium was incubated for 5 h at 4°C, with 3.5 × 107 magnetic beads carrying an anti-HLA class II monoclonal antibody in 500 μl serum-free culture medium, under mild agitation (Dynabeads-HLA class II, Dynal-Invitrogen).
Similar(56)
Complexes 1 and 2 were synthesized in basic medium and under mild hydrothermal conditions.
Animals were killed by cervical dislocation, and interscapular fat depots were rapidly excised and washed in phosphate-buffered saline, minced and digested for 45 min at 37°C with 2 mg/ml collagenase A (Roche) in the presence of 20 mg/ml bovine serum albumin (Sigma) in Dulbecco's modified Eagle's medium (DMEM) under mild agitation.
The DO meter was calibrated by setting the output reading to 100% value, which was obtained by purging Dubos medium with air, under mild stirring, for ∼10 minutes to attain the saturation limit for DO.
For the bark fraction, the glucose yield was relatively high even without pretreatment and did not increase very much after pretreatment under mild or medium conditions (Table 4).
For pathogen challenges, bacteria were grown overnight at 28°C under mild shaking in liquid King B medium.
Ten mL Agrobacterium cultures were grown overnight under mild shaking at 30°C in regular Luria-Bertani medium containing 25 μg mL-1 rifampicin, and 50 μg mL-1 kanamycin when necessary.
Taken together, use of RehMBEL1391 further provides in silico confirmation that pH value of fermentation medium significantly influences the polymer yield and its yield is optimal under mild basic condition.
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