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However, only a very small portion of rZZ AP is generally secreted into the aqueous medium under conventional cultivation procedure.
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The tissue was mechanically dissociated and enzymatically digested after neurosurgery, then passed through a 70 μm strainer and cultured in Dulbecco's minimum essential medium supplemented with 20% FCS under conventional culture conditions [ 26].
Flies were maintained on conventional cornmeal-agar medium under a 12 hr light/dark cycle at 25°C and 60% relative humidity.
At first, HUH-wt, HUH-PAS, and HUH-REISO cells were pre-cultured under conventional conditions or under a thin layer of solid medium ("agarose overlay", Figure 9D-F) for six weeks.
For more detail characterization of the bacterium, the changes of cell growth and pH in the medium were also measured according to the culture temperature since the temperature of the hands was mostly affected under conventional environments.
Contamination of hESCs by Neu5Gc was confirmed following culturing under conventional conditions with mouse embryonic fibroblast (MEF -derived feeder cells and knockout seruMEF -derivedt (KSR)-supplemented medium [7], [8].
All fly stocks were maintained on conventional cornmeal-agar-molasse medium under a 12-hr-light:12-hr-dark cycle at 18°C or 25°C.
All fly stocks were maintained on conventional cornmeal-agar-molasses medium under L D 12 12, RH = 70% and 25°C.
Under conventional economic logic, Mr. León is uncompetitive.
Nanofluid is employed as working medium for conventional circular heat pipe.
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