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Each algal strain was cultured in a CA medium under constant light at ca. 1500 lux of natural white fluorescent light and 24°C [7].
T. castaneum beetles were purchased from Biologische Bundesanstalt (BBA); Berlin, Germany, and were reared in glass containers provided with growing medium under constant conditions of 30°C and 70% relative humidity at L 16: D 8 hrs photoperiod.
For callus induction, minced seedlings were incubated in RM28 medium under constant light.
SEM image of a seeded scaffold incubated in growth medium under constant agitation.
Respiration of isolated liver mitochondria was determined at 37°C in 2 ml of the mitochondrial respiration medium under constant stirring.
To assay luciferase activity, C. reinhardtii cultures were grown in liquid TAP medium under constant illumination (55 μE m − 2 s − 1) to a final cell density of 3 6 × 10 cells ml − 1.
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Interesting analytical results, in terms of sensitivity and temporal reproducibility, in alkaline medium and under constant applied potentials (i.e., −1.45 and −0.6 V vs. SCE), were obtained.
Total RNA was isolated from C. reinhardtii cells grown in TAP medium and under constant light.
20 ml overnight cultures were diluted in 500 ml LB medium and grown under constant shaking at 37°C to an OD of about 0.6.
All fungi were grown in the same medium and kept under constant conditions to avoid influence of biotic and abiotic factors on the expression of secondary metabolites.
Cells of the strain 137c were maintained on Tris Acetate Phosphate (TAP) agar or grown in liquid TAP medium at 23°C under constant illumination of ~100 μE/m·sec-1.
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medium under phototrophic
medium under anaerobic
medium under non-selective
medium under physiological
medium under initial
medium under high-temperature
medium under hypoxic
medium under microaerophilic
medium under recommended
medium under stationary
medium under static
medium under normal
medium under various
medium under conventional
medium under intensive
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