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Both medium types were replaced twice a week.
All the above-mentioned cell culture medium types were supplemented with 10% foetal calf serum (FCS), 2 m M L-glutamine, penicillin (100 units ml−1) and streptomycin (100 μg ml−1) (GIBCO).
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In an effort to increase the yield and quality of the recombinant product, common parameters (culture temperature, medium type) were varied, followed by the bioreactor pilot expression of the recombinant enzyme (Supplementary data, Figure S1).
One column of each aggregate or medium type was planted.
It is reported that temperature and medium type are two important factors that control the particle size [ 37, 38], but in the present study the effect of temperature was rare.
The time to flowering and maturity from the extra-short to medium-duration types were simulated well, explaining 96 and 92% of the variance (RMSD=4.3 and 9.8 days), respectively.
The two later-stage tumour types were of medium size (T2/T3), homogeneous differentiation and lymph node involvement (N1 N2c).
In a recent paper by Suggett et al. (2008) H2O2 production (and release to the medium) by two Symbiodinium types was measured at varying temperature and light intensities [41].
The culture medium for both cell types was replaced twice a week.
The conditioned medium from both cell types was mixed with RPMI-1640 10% FBS in a 1 : 1 ratio.
The conditioned medium from both cell types was mixed with DMEM-10% FBS in a 1 : 1 ratio.
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