These cells were diluted 250-fold into the same medium, transferred to polyethylene bulbs, sealed with no air space and placed inside pressure vessels.
For example, the magnitude of RIBE for DDR activation in WI38 human fibroblasts was 2.5-fold with medium transfer, about 3-fold with CMRA-staining cell co-culture studies, and 3.7-fold with α-particle IR exposures [5].
For example, the magnitude of RIBE for DNA damage response (DDR) activation in WI38 human fibroblasts was 2.5-fold with medium transfer, about 3-fold with CMRA-staining cell co-culture studies, and 3.7-fold with α-particle IR exposures [5].
Seven-day-old seedlings grown on MS medium were transferred to the medium with various NO3 −, Ca2+ and K+ concentrations, and grown for 14 days in a growth chamber at 24 °C under 8 h of darkness and 16 h of light at 150 μmol photons m−2 s−1.
To quantify the frequency of the pyrG loss events, single colonies from I- SceI-induced medium were transferred into minimal medium with and without uridine.
For induction with 5-MT, fungal mycelium from 4-day-old liquid culture in Czapek Dox (CD) medium was transferred in CD medium supplemented with 5 mM 5-MT and incubated for 8-h.
For studying the effect of 2,4-D, exponential cells grown in MM4 medium were transferred to fresh medium supplemented with 0.45 mM 2,4-D, and grown for 1 h at 30°C.
For caulonemal filament induction assays, moss colonies were grown for 10 days in BCDAT medium, then transferred to BCDAT medium supplemented with 5 g/L glucose and grown in the dark for 10 additional days.
We and others reported that the RIBE in human adult differentiated somatic cells can be readily observed both with medium transfer and cell co-culture protocols [5], [13], [14].
With the other types of human cells, we observed the magnitude of RIBE to be no less than 2-fold over sham-exposed cells with bystander medium transfer technique [14].
