Sentence examples for medium to stop from inspiring English sources

As 1 μL of stop buffer was added into the medium to stop the reaction, shRNA from NPs was extracted according to the above method and the products were analyzed by agarose gel electrophoresis.

Passages were obtained by dissociation from plates using 3 min trypsin-EDTA treatment and complete hNCC medium to stop the reaction.

Incubation of vesicles with the radiolabeled nutrient was continued for time periods from 1 to 60 min after which a known volume of this incubation mixture (20 µL) was withdrawn and plunged into 2 mL ice-cold stop solution (generally an isotonic choline chloride medium) to stop the uptake process.

(A ): growth at 37°C for 10 min, then 120 min induction (addition 1% galactose); (B ): induction for 50 min with 1% galactose at the permissive temperature (25°C), then cells were washed into dextrose containing growth medium (to stop transcription) and grown for 10 min further at 25°C before temperature shift to 37°C for 60 min. (C and D ) As A and B respectively, except expressing GFP-Sso1p.

The range of electrons may be taken as the greatest distance of penetration in a given direction, or the minimum thickness of the medium required to stop all electrons.

HeLa and Caco-2 cells were collected by trypsinization using trypsin/EDTA buffered solution for 5 min at room temperature, followed by the addition of 5 mL of serum-enriched medium (CM) to stop trypsin action.

At 2 min after trypsin digestion, complete medium was added to stop the reaction.

Thereafter, growth medium was added to stop the dispase activity by dilution.

The medium was removed to stop the reaction and then 150 μL dimethylsulfoxide (DMSO) was added.

Rinsing with 10% fetal bovine serum (in L-15 medium) was used to stop the trypsin reaction.

The cell pellet was then agitated for 45 sec, and 40 ml of prewarmed serum-free RPMI medium was added to stop the reaction.