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Beads were returned to the reactor and the same amount of fresh CM at 30 °C was added, stirring the reaction medium, to start a new fermentation cycle.

After 1 week of induction the culture was centrifuged, the conditioned medium was harvested and the cells were suspended in fresh induction medium to start another round of induction.

For the determination of growth rates, a pre-culture was prepared incubating C. albicans isolates over night at 30°C in liquid SD (0.17% of YNB, 0.5% of (NH4 2SO4, 2% of glucose) and YPD (1% yeast extract, 2% peptone, 2% glucose) media and then a dilution was prepared in fresh medium to start with a OD600 of 0.05.

After 20 h of continuous circulation, the broth was replaced with fresh P2 medium to start the ABE fermentation.

The medium was changed to complete medium without FCS and HAT and after 16 h incubation time, native or modified HDL, LDL or BSA was directly added into the medium to start the experiment.

Similar(55)

For long-term growth curves, strains were first grown to saturation in yeast extract/peptone/2% glucose (YPD) medium, after which, to start the growth curve, they were diluted 100-fold into a total of 5 ml of yeast extract/peptone medium containing either 2% xylose or no carbon source.

It seemed to me that if there was any medium in which to start playing with Sophocles's trilogy, it was opera.

During the contention phase, each station senses the medium in order to start the frame transmission.

I fried the pancakes -- about a tablespoonful of batter each or a bit more -- in neutral oil, starting them over medium-high heat to start them browning, then lowering the heat to medium to cook them through (for the second batch there was no need to increase the temperature for the initial browning).

At an OD600 between 0.3 − 0.5, a sample was withdrawn to inoculate the bioreactor (700 ml) or batch culture (500 ml Erlenmeyer flask containing 50 LBT medium) to a starting OD600 of 0.005.

Overnight cultures of planktonic cells in Todd Hewitt Broth were diluted in the biofilm medium to a starting OD of 0.05 and biofilms were grown for 3 hours under anaerobic conditions (80% N2, 10% CO2, 10% H2) in a chamber (Don Whitley Scientific, Shipley, England).

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