Sentence examples for medium to repress from inspiring English sources

In experiments utilizing strains containing plasmids under the control of the nmt1 thiamine repressible promoters, 20 µM thiamine was added to the medium to repress protein expression.

One mg/ml of doxycycline was added to the BJAB-tTAE1 culture medium to repress the expression of EBNA-1.

At increasing time intervals, we overlaid the GAL plates with either GLU-URA or GLU+URA medium to repress BRCA1 expression.

Co-propagation of the LacIQ plasmid and pAAT24 or pTTA24 improved plasmid recovery, which was also consistent with the result of adding 5% glucose to the medium to repress lacZ gene transcription (data not shown).

For canavanine resistance assays, single yeast colonies (at least 12 independent colonies for each experiment) that had been grown overnight in glucose medium to repress expression from the GAL1 promoter were diluted 1 100 into galactose-containing medium and grown for 2 days at 30°C before serial dilutions were plated onto canavanine-selection or viability plates.

Cells were grown in medium containing galactose, then the culture was split and one half was transferred to medium lacking galactose to repress Scc1 expression.

YEPD (2% (w/v) yeast extract, 2% (w/v) glucose and 0.1% (w/v) peptone) medium and CM (complete medium) [ 29] were utilized to repress the P alcA completely and partially, respectively.

A high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, an important event in regulating curli and cellulose production [ 19].

Enolase is a glycolytic enzyme whose transcriptional level is constitutive in glucose-containing medium [ 4], a situation which simultaneously serves to repress endogenous cellulases.

Cells were grown at 32°C in Edinburgh Minimal Medium (EMM) [36], adding either uracil at 0.25 mg/ml to induce Purg1, or 15 µM thiamine to repress the nmt1 promoter.

We always want to repress it.