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Medium was aspirated and replaced with fresh medium to remove non-adherent cells every 2 to 3 days.
In ultrasonic machining a vibrating tool causes a liquid abrasive medium to remove material.
Iron modified materials have been proposed as a filter medium to remove arsenic compounds from groundwater.
The wounded monolayers were washed with the growth medium to remove the debris and exposed to conditioned medium.
The cells were rinsed with medium to remove traces of serum, which may inhibit action of trypsin.
The cell viability was measured by refreshing the culture medium to remove the stressors and cultivated for 2 h.
After washing with culture medium to remove cell debris, the cells were allowed to migrate for 24 h, followed by observation under an Olympus CKX41 optical microscope.
The medium was then aspired, and the cell layer was rinsed three times with growth medium to remove any traces of uninternalized GAuNPs.
Cells were then rinsed twice with serum-free medium to remove cellular debris.
Cells were harvested and washed with pre-warmed medium to remove extracellularly present bacteria.
Dorsal integuments were severed carefully with sterilized scissors and washed with culture medium to remove haemolymph.
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