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Using an alkaline medium to prepare exfoliated IAGs could be an important shift in the preparation of these materials.
The copolymer was synthesized and suspended in an aqueous medium to prepare its core shell particles.
Firstly, one loop of P. aeruginosa was added to 25 mL of broth culture medium to prepare bacterial suspension.
In this study, monodisperse polystyrene nanospheres were prepared by dispersion polymerization using alcohol as reaction medium to prepare colloidal clusters of the latex beads.
One vaginal suppository tablet (water soluble) was dissolved in 44 mL serum-free RPMI1640 culture medium to prepare a drug stock solution of 3.95 × 104 µg/mL, and was passed through a 0.22 µM membrane filter for sterilization.
One of the methods that is used for NISV preparation is microfluidics in which the lipid components dissolved in organic phase are mixed with an aqueous medium to prepare the particles through self-assembly of the lipids.
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90 mg/l ampicillin was added to Medium C to prepare Medium M [41].
A single colony from each respective NA culture was then used to inoculate 5 mL sterile mineral salt medium (MSM) to prepare seed cultures.
It has been reported previously that the growth medium used to prepare C. albicans inoculums affects the ability of strains to gain an initial invasive advantage immediately after injection into host, therefore influencing virulence (Odds et al., 2000).
Lyophilized cells were reactivated for 24 h at 75 °C and inoculated in new medium 640 to prepare stock cultures of 1 ml containing 30%% (w/v) glycerol which were then stored at −80 °C.
To obtain the extracts, three specimens from each considered sample were immersed in DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Non-interfered complete medium was considered to prepare the control.
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