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When using cDNA as template we camplified intragenic regions of each gene of the cluster (Fig. 5A and B, bars and lanes 2 to 7), confirming that these genes are expressed in cells tat have grown in rich medium to late exponential phase.
Year-to-year correlations of medium to late season DSI assessments showed R values of 0.59, 0.69, and 0.55 for 2005/2006, 2006/2007, 2005/2007 comparisons, respectively.
Macbeth is a medium to late maturing variety that is also lodging resistant and has good yield, high oil content and good oil quality.
Wild-type and mutant cells were grown in 100 ml of modified VM cellobiose medium to late log phase (optical density at 600 nm of 0.65 to 0.85).
Population J3 (6 accessions) regrouped the medium to late accessions from the South Central Coast and Southeast regions, with long, large non-glutinous grains.
C. reinhardtii wild type strain 137c was grown in TAP liquid medium to late log phase (approximately 7 days), with subsequent cell harvesting by centrifugation (2060 g for 10 minutes at 4°C).
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The highest transformation efficiency of butanol-tolerant GRSW2-B1 at 5.17 × 103 CFU (μg DNA -1 was achieveDNA -1 the competent cells wase prepachievedm cells growhenn LB medium thelate-exponential phase with OD600 of 0.6, and washed with icompetentSMG buffer.
The YHB1-GFP construct and wild type cells were grown in YPD medium to the late logarithmic phase.
Next, to better evaluate RAP-011 effects on later stages of erythroid differentiation, CD36+ cells were used as a starting point and co-cultured with LTC-IC culture in liquid medium to support late stage erythroid precursor growth and differentiation.
Carbon and oxygen isotopes were analyzed in medium to large herbivores from three late Miocene and three Pliocene local faunas comprising a total of 13 and 7 genera, respectively, and 59 and 42 individuals.
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