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CPA solution was slowly diluted in fresh medium to inhibit osmotic damage.
Accordingly, amoxicillin (AMX) was loaded into the coronas as a medium to inhibit the bacterial viability of Escherichia coli and Staphylococcus aureus.
After autoclaving, 40 μg ml−1 chloramphenicol and 33 μg ml−1 rose bengal were added aseptically to the medium to inhibit growth of bacteria and fungi, respectively.
We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds.
Dispersed cells were washed in complete medium to inhibit the trypsin then centrifuged to pellet the cells.
In order to block RA signaling, we used AGN193109 (10−6 M) in the culture medium to inhibit activity of all RARs [30]; Supplementary Figure 1).
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The concentration of the phosphate salt in the medium seemed to inhibit the growth so as to extend the culture period.
In agreement with our hypothesis, β-secretase inhibitor treatment counteracted the APP-conditioned medium ability to inhibit HIPK2 binding to DNA and re-established HIPK2 recruitment onto HIF-1α promoter (Figure 2a, right panel).
TNF-a was shown to stimulate growth as well as to induce extensive branching and alveolar morphogenesis of isolated rat mammary epithelial cells in primary culture, and, under optimal medium conditions, to inhibit the accumulation of casein proteins [ 102, 103].
NIH/3T3 cells were either starved for 24 hrs using starving medium (culture medium w/o FCS) to inhibit proliferation or cultured in full culture medium to promote mitosis.
Fifteen mmol/L 3-amino-1,2,4-triazole (3-AT) was added to the growth medium each time to inhibit low levels of His expression in a leaky manner when a potential candidate was validated.
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