The live EBs were grown in conditioned medium to induce differentiation towards cardiac phenotype.
The cells were then centrifuged and resuspended in 5 mL BMMY medium to induce expression for 4 days.
When the OD600 reached at 0.2, 1 mM IPTG was added into the medium to induce the protein.
After that, 0.2 mM IPTG was added to the medium to induce enzyme expression, and the culture continued to grow at 20°C for 20 h.
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The nutrient rich mucin protein complex, in addition to antibiotics, have been shown to enhance biofilm formation [22, 35, 36] and both were included in the medium to induce robust biofilm formation and provide a simulated clinical environment [19].
We switched the different transfected TE671 cells to a low serum medium to induce differentiation.
TS cells grown to confluence in TSMFH medium were switched to TS medium to induce differentiation.
The cells were released from G1 arrest in methionine-free medium to induce CLN1 or CLN2 expression.
The pharmacological agent N-methyl-D-aspartate (NMDA) was added to the ringer medium to induce bursting activity (10 µM).
Cells were grown in galactose medium to induce DSB formation, followed by transfer to glucose medium to terminate HO expression.
