Sentence examples for medium to final from inspiring English sources

After that, the cells were resuspended in the YNB medium and inoculated into the YNM medium to final density for cultivation (OD600 = 1.0).

(2) The concentration of extracellular K+ was increased by adding potassium gluconate (Sigma-Aldrich) to the medium to final concentrations of 10 80 mM.

(3) The ATP-sensitive potassium channel (KATP ) openers pinacidil and diazoxide (Sigma-Aldrich) were added to the medium to final concentrations of 1, 10, or 100 µM from 10 mM stock solutions in ethanol.

CM was then mixed with fresh medium to final proportions of 30%, 50 % and 80.

Dilutions in culturing medium to final concentrations of 10 0.5 μmol/L were done immediately before use.

To optimize the concentration of xylose, we added xylose to the growth medium to final concentrations of 0 to 3.0%.

Subconfluent cells were treated with adriamycin (ADR) diluted into the medium to a final concentration of 1.5 μg/ml, bleomycin (Bleo) diluted into the medium to a final concentration of 120 μM, zinc chloride (ZnCl2) diluted into the medium to a final concentration of 100 μM, or X-ray irradiated with 40 Gy, for the indicated period of time.

When needed, ampicillin was supplemented into LB medium to a final concentration of 100  μg mL−1, and kanamycin was added into liquid SVO medium and SVO plates to a final concentration of 150 and 250  μg mL−1, respectively.

4-OHT (Sigma-Aldrich), diluted in ethanol, was added to the crypt culture medium to a final concentration of 500 nM for at least 12 h.

Formaldehyde was added directly to tissue culture medium to a final concentration of 1% and left for 10 min at room temperature on a shaking platform.

For N-acetyl-p-aminophenol (APAP) metabolite quantification, APAP was dissolved directly in cell culture medium to a final concentration of 1 mM.