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Extracts were prepared from the wild-type strain and the ΔptsP, ΔptsO and ΔhprK mutants grown in rich medium to exponential phase (OD600 = 0.8).
Yeast strains were grown overnight in SD-Ura medium to exponential phase (OD600 of 0.6 0.8). 100 nM rapamycin was added to the cultures and cells were harvested at various time points.
Strains were grown in YPD medium to exponential phase.
Briefly, fission yeast cells were grown at 25°C in YE5S medium to exponential phase.
Fission yeast cells were grown at 25°C in YE5S medium to exponential phase.
Cells were grown in YES medium to exponential phase and imaged on a slide harboring an agarose patch containing YES medium with 3% glucose.
Similar(52)
Yeast strain MH272-1da [41] transformed with constructs as indicated in the result section was cultivated in 50 mL SD-URA medium to the exponential growth phase.
Y. pestis WT strain 201 and the Δhfq::KmR mutant were grown at 26°C in LB medium to middle exponential phase and then transferred to 37°C for 3 hr.
When using cDNA as template we camplified intragenic regions of each gene of the cluster (Fig. 5A and B, bars and lanes 2 to 7), confirming that these genes are expressed in cells tat have grown in rich medium to late exponential phase.
The cells were split on a 2-2-3-day schedule at 2-3E5 cells per ml, in the appropriate pre-warmed medium to maintain exponential cell growth.
For induction of the GAL1 promoter, cells were pre-grown in a medium containing 2% raffinose, transferred to 2% galactose medium for overnight growth, and diluted into fresh galactose medium to obtain exponential cultures.
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