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The cells were incubated at 37 °C for 4 h to allow the cells to diffuse into and adhere to the scaffold before the addition of 100 µl of culture medium to each well.
Osteoblast-like MG63 cells (3 × 104 cells/mL) was seeded onto a new 24-well plate with 1 mL fresh medium to each well.
This was followed by the addition of 100 μl of MTT (0.5 mg/mL) prepared in serum-free medium to each well and incubation for 4 h at 37°C.
We added a set volume of cell culture medium to each well, and supplemented the medium with the Alamar Blue compound (resazurin).
KC cells in 96 well plates were prepared immediately before the titration with the addition of a cell-suspension (approximately 1×106 KC cells in 150 µl Schneider's growth medium) to each well.
CH (0.06 2.5 mM) was added in a small volume of incubation medium to each vial.
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Because they use a shared medium to communicate, each node will be able to utilize only a fraction of the underwater channel bandwidth.
we're already seeing the candidates use the medium to comment on each other".
Every 24 h, samples were collected from each medium to check the cell biomass and absorbance at OD600.
A separate analysis was conducted in each medium to estimate genetic correlations.
Culture supernatants were harvested and fresh medium added to each well every 3 4 days.
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