There may have been differences in respondents based on the medium used to complete the survey (electronic vs paper-based).
At the end of transfection (i.e., 0 h timepoint), the medium was changed to complete medium containing 10% FBS.
Give it a good, all-over misting with a medium-hold hairspray to complete the look.[30].[30]
For the drug assays, the antimalarial drugs were added at different concentrations to complete medium.
Peripheral Blood Monocytes or THP-1 cells were exposed to complete medium in the presence or absence of carrageenan for 36 h.
Cells were returned to complete medium for 20 min and then fixed with ice-cold methanol.
Next, the cells were deprived of serum for 48 h and then returned to complete medium for an additional 24 h.
Fungus grown on OM plates were transferred to complete medium (CM: 10 g sucrose, 6 g yeast extract, 6 g casamino acid and 1 mL trace elements/L) plates and subsequently to complete liquid medium.
The suspension was removed and sequential rinses were performed with 0.8 ml aliquots of medium to ensure complete removal of the cells.
Naturally occurring amino acid isotopic variants were eliminated from the serum supplementing the growth medium by dialysis, and cells were passaged a minimum of five times in the labeling medium to ensure complete incorporation of the labeled amino acid.
