Sentence examples for medium three times from inspiring English sources

Thereafter, the adherent ependymal cells were fed by the replacement of 2 ml of medium, three times a week [24].

The adherent cells were briefly and gently washed with complete medium three times and some wells were assayed immediately by adding 100 µl of gel loading buffer to each well and run on a gel or counted in a scintillation counter.

Flies were transferred to a fresh medium three times a week.

BNP was added to the medium three times a day as previously described [ 13].

The next day, the cells were washed with complete medium three times.

The SHIME reactor was fed carbohydrate-based medium three times per day to provide digested nutrition for the colon microbiota.

Cells were washed with Glasgow's medium four times to remove unbound virus before seeding into 24-well plates.

To wash out stimulants or inhibitors in compounds contained in the culture medium, the culture medium was replaced with an equal volume of complete medium four times.

Animals were fed ad libitum with Desmodesmus subspicatus (final concentration: 15.5 × 104 cells per ml culturing medium) six times a week.

Live retinal cultures were incubated with 20 nM tetramethylrhodamine, methyl ester (TMRM), and MitoTracker red CM-H2XRos (MitoTracker) for 20 min and washed with culture medium three and six times, respectively.

After washed with RPMI 1640 medium five times, the adherent cells were cultured for 6-8 days on flat-bottom 6-well plates in RPMI 1640 (Gibco) supplemented with 10% FCS (Biowittaker), L-glutamine (Gibco; 2 mmol/L), 1% penicillin-streptomycin Seromed), GM-CSF (50 ng/ml) and IL-4 (25 ng/ml).