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Kim and colleagues (2001) showed that when SCs are cultured in PDGF-free medium, they become growth-arrested, with a diploid (G1) content of DNA.
In the specific examples considered above, when the p Ka of the heterocycles (2, 3, and 6) is close to the pH of the aqueous medium, they become deprotonated or protonated (ionized) and, therefore, hydrophilic.
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Fully developed embryos were cultivated in WPM medium until they become plantlets.
Wall protein precursors may be washed out into the medium before they become covalently linked to the glucan-chitin network, especially in shaken cultures.
Add the bell peppers and simmer on low - medium heat until they become tender and have released their juices.
Twice daily, the explants were fed with 20 µl B/D medium until they became adherent (maximum of three days).
It is after sometime, when they become medium sized, that they start looking at a vision of what they want to be.
In Mel-mix medium melanocytes proliferate rapidly, they become bipolar and they lose their pigmentation after a few weeks in culture.
But both are expanding where they can, snapping up small and medium-sized producers as they become available.More consolidation would certainly give steel firms extra clout when negotiating ore and coke prices.
Place the potatoes in a pot of water and boil them over a fire until they become medium-soft.
Throw the cloves, cinnamon, chili, and thyme into a pot and toast them over medium-low heat until they become aromatic, about 3 - 5 minutes.
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