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The test was performed by adding known amounts of the 9 standards at low (80% of the known amounts), medium (the same as the known amounts) and high (120% of the known amounts) levels.
Bacterial cells were grown at 37°C with shaking at 220 rpm in LB broth (Lennox) medium or minimal medium (the same as M9 but without NH4Cl; 12.8 g Na2HPO4 7H2O, 3 g KH2PO4, 0.5 g NaCl per liter).
Similar(58)
The composition of the latter medium was the same as the growth medium but with reduced amounts of glucose (10 g l−1) and yeast extract (0.25 g l−1) and the nitrogen source was NH4NO3 (0.3 g l−1).
The differentiation medium was the same as the hESC medium except that the KOSR was replaced with 20% non-heat-inactivated FCS.
The EB culture medium was the same as hESC culture medium but without bFGF and hLIF.
Regeneration medium is the same as callus initiation medium except with 2 mg/l (9.3 μM) kinetin rather than 2,4-D, and supplemented with 1 mg/l bialaphos.
The fermentation medium was the same as the seed culture medium except the carbon sources.
The constituents of the maintenance medium were the same as those of the growth medium except that only 2 % FBS was added.
The other ingredients in RSHH medium were the same as that in the control group.
K+-free medium was the same as normal Hanks' solution without 5 mM KCl.
The rooting medium is the same as above except with 2.0 mg L-1 6-benzylaminopurine and 2.0 mgL-1α-naphthlcetic acid.
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