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In a companion paper, Jakubowicz and Levin [2] showed that in a constant velocity medium the method is equivalent to the classical one-step 3D migration.
She controls the medium, the method and the message.
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R. litoralis and R. denitrificans were tested for intracellular production of glycogen when grown in complex medium (modified 70% MB, see Additional File 5) and in mineral medium using the method described by Bourassa and Camilli [ 57].
In other words, it is neither the medium nor the method that counts during the transfer of information, but the fidelity of the transfer.
The impact was considered as "medium" when the method of treatment delivery was changed (e.g. radiation treatment volume and/or dose fractionation) [ 17].
Sterigmatocystin was extracted from 10 mL aliquots of filtered growth medium using the method described by Keller et al. with the following modifications.
Chitinase activity (beta-1,4-glucosamine polymer degradation) was tested in chitin minimal medium by the method of Chernin et al. (1995).
Sterigmatocystin was also extracted from three 16 mm plugs taken from conidiating colonies grown on solid minimal medium using the method described by Keller et al. with the following modifications.
In (B ), restriction to the second season was achieved by using the glucose-depleted acetate-rich solution, produced by the full-batch coculture, as growth medium (see the Methods for details).
Screening of chitinase activity was performed using chitin detection medium according to the method given by Agarwal and Kotasthane [ 33].
Qualitative differences between laboratories could be explained by differences in thiol-reagent content of assay medium and by the method of homogenization.
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