Sentence examples for medium the final from inspiring English sources
In the 2% differentiation medium, the final concentration of the antisense PMO was 10 µM for a single sequence, 20 µM for two sequences, and a total of 30 µM for three or four sequences.
Compounds were dissolved in DMSO and further diluted with RPMI 1640 medium (the final DMSO concentration did not exceed 0.5% which did not affect parasite growth).
To evaluate the effect of laccase treatment on yeast growth, prehydrolysates were diluted to 75%, 50%, and 25% of original concentration by 4× YNB medium (the final concentration of YNB medium was 1×) [ 55].
After 72 h of exposure, 10 μL of the MTT labelling reagent was added to the medium (the final concentration of MTT was 0.5 mg/mL), and the cells were incubated for an additional 4 h.
By immediately diluting the 1 M NaCl eluate in Luria broth (LB) medium, the final NaCl concentration of the eluted cell suspension was reduced to approx. 0.4 M NaCl.
The mixture of the five epimutagens, DEP, Hg, cotinine, Se, and S-421, dissolved in HNO3 and EtOH was added to culture medium, and the final concentrations in culture medium were 0.0025% and 0.1%, respectively.
Na2S·9H2O (0.50 g/L) was used to reduce medium and the final pH of the basal medium was adjusted to 7.2.
Resistant cells were routinely grown in selective DHFR medium lacking glycine, hypoxanthine and thymidine (-GHT medium; Gibco), the final products of dihydrofolate reductase (DHFR) activity.
LCA was added to growth medium at the final concentration of 50 μM immediately following cell inoculation into the medium.
Unexpectedly, the growth rate of P. purpureum was rather slow in F/2 medium and the final biomass concentration obtained was only 2.58 g/L.
In contrast, EDTA-permeabilized wild-type P. chlororaphis did not hydrolyze the 0.5% (w/v) raffinose in the medium, and the final biomass yield was 0.26 g CDW/l.
