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Cells were seeded at 5 × 10/cm2 in stem cell proliferation medium the day before the addition of SAGM and then were differentiated for eight days and medium was changed after four days.
LOVO cells were seeded in antibiotic-free medium the day before transfection.
For MDA-MB-231 cells, growth medium was replaced with serum-free AIM-V (Invitrogen) medium the day after seeding.
E. coli were sub-cultured in liquid LB broth medium the day before the experiment was performed.
For RNA collection, 7,000 mESCs per well of a 96-well plate were plated into antibiotic-free ES medium the day before transfection.
For reporter assays, 10,000 mESCs per well of a 96-well plate were seeded into antibiotic-free mESC medium the day before transfection.
Similar(46)
The medium was replaced by fresh complete growth medium the next day and then every second day until cells reached confluence.
Y-27632 was added to the medium on the day of passage.
In this way he managed to save thousands of beautiful Jewish folk songs, klezmer dance tunes and religious melodies on wax cylinders, the medium of the day.
When I felt a video or cartoon conveyed some idea more clearly than didactic lecturing, I included that medium in the day's lecture.
In turn, the space story helped make him famous and television the dominant news medium of the day.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com