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As a general trend, for W V O thin films with low vanadium content cycled in protonic medium, the cell capacity, the contrast and the coloration efficiency decrease as the vanadium content increases.
But in the supplemented medium, the cell viability went up even up to 12% (v/v) ethanol.
However, when the sample was incubated in Bristol medium containing 10 mM TCEP·HCl for 1 h at room temperature followed by rinsing with Bristol medium, the cell density decreased noticeably.
For infection of confluent BHK-21 cell monolayers with supernatants of FMDV-infected DCs in liquid medium, the cell culture medium was removed and the supernatant added onto the cell monolayer.
Within the first 2∼3 days, about 20 30% of cells were lost in the normal dense culture and removed by changing the medium; the cell culture usually became stable thereafter.
These results suggest that in SC medium the cell cycle arrest response of bem1Δ cells to DOX is more rapid than that observed in YPD resulting in a clear G1 arrest.
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It does not matter what type of medium the cells were grown in prior to UV irradiation (A94).
When grown in osteogenic medium, the cells expressed alkaline phosphatase (ALP) and osteocalcin mRNA.
After the removal of the cell medium, the cells were washed by 70% cold methanol for 15 minutes on shaking.
When they put human macrophages in the lower compartment filled with cell culture medium, the cells rose toward the membrane.
After removing the medium, the cells were washed three times with PBS in order to detach the free csMSN.
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