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Under optimized medium, the average inulinase activity reached 205.63 U/g initial dry substrate.
The Caco-2 colon adenocarcinoma cell line retained the greatest number of radioactive counts after a two-hour incubation and subsequent washes with complete medium, the average value of triplicate wells equaling 298,639 cpm per 10,000 cells.
In DMEM F-12 medium the average size and zeta potential of CeO2 NPs was shown to be 177.4 ± 0.23 nm and −13.7 ± 0.25 mV, respectively, with polydispersity index (PDI) 0.248 (Table 1).
We found that in E6/E7-expressing cells grown in a folate-free medium, the average fork distance was significantly shorter than in E6/E7-expressing cells grown in a normal medium (P < 5 × 10−3) (Appendix Fig S4B).
After 4-6 days in the TSI medium, the average cell size significantly increased as well, from 19.0 ± 4.2 μm (mean ± SD, n = 111) in diameter at day 0 to 25.6 ± 12.5 μm (n = 124) at day 6.
The average replication rate of the E6/E7-expressing keratinocytes in the normal medium was 0.79 Kb/min, whereas in the folate-free medium the average fork rate was significantly reduced to 0.58 Kb/min (P < 1.5 × 10−5) (Appendix Fig S4A), indicating that folate deficiency significantly enhances the effect of E6/E7 oncogenes on cellular DNA fork progression.
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On Potato Dextrose Agar medium (PDA) the average growth rate per day of the colony mycelium was faster and also higher under the longer light period (mean 1.25±0.01 SE cm/day), compared to 1.11±0.11 cm/day for the 3-hour photoperiod (t test, n = 12, P<0.018) and the mycelium was notably denser with more aerial mycelium (Fig. 3A).
On Water Agar medium (WA) the average growth rate per day of the colony mycelium for five days was higher under a 12-hour light cycle (mean 0.52±0.03 SE cm/day) than under a 3-hour light cycle (0.38±0.03 cm/day, t test, n = 12, P<0.004).
Again as expected, for samples with medium signals, the average CV was higher at 28% (range 22%42%%).
Doubling times in YPD medium represent the average of four biological replicates; the average difference ('dif') in growth rate is indicated.
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