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In acid medium, the addition of Rh to Pt catalysts increases the CO2 yield by facilitating C C bond cleavage; however, the obtained currents do not increase [19, 20, 21].
In Cu-replete medium, the addition of Ni at 25 µM does not induce the CYC6 promoter at all while Ni at 50 µM results in a weak induction (0.2× PSAD).
In high glucose medium the addition of the U0126 inhibitor for 12 hours caused a small decrease of p-Bad and the Bcl-2 family member Mcl-1 protein levels together with a slight increase in the amount of BimEL.
Furthermore, our data showed that in high glucose medium the addition of U0126 (10 µM) or sorafenib (data not shown) had no effect on AMPKα phosphorylation in all BRAF mutant cell lines (Fig 5A).
Interestingly at glucose concentrations normally used in the cancer cell culture medium the addition of L-lactate resulted in the inhibition of prostate cell proliferation.
On the contrary, in low glucose medium, the addition of 5 mM L-lactate sustained cell growth respect to untreated cells.
Similar(52)
Media were routinely supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin. MCF-10A cells were cultured in endothelial cell basal medium with the addition of medium supplements provided by PromoCell (Heidelberg, Germany) plus 100 ng/ml choleratoxin.
In addition, we cultured cells in normal medium or sodium-free medium before the addition of cationic carriers.
When cultures became 100% confluent, medium was changed to ITS medium with the addition of 500 ng/ml Noggin and 10 µM SB431542 for 7 days.
For differentiation into embryoid bodies (EBs), ESCs were trypsinized and transferred to ultra-low attachment plates into 15% FBS containing medium without the addition of LIF and medium was changed every second day.
HAEC were pre-treated with respective pharmacological inhibitors or vehicle (DMSO) for 45 min in serum-containing medium before the addition of fresh FCS-medium containing LPC.
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