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Their simulations showed unexplained delays during packet transmission (TX) over the radio medium that were not observed during similar experiences on physical motes.
The swelling characteristics of beads were determined by immersing them in dry state into conical flask containing 40 ml of release medium that were incubated at 37°C under shaking at 150 rpm.
Yellow colonies from TCBS medium that were suspected as V. cholerae were subcultured onto LB agar, and then tested for oxidase (1% tetramethyl-p-phenylenediamine; Sigma) and subjected to the string test (0.5% sodium deoxycholate; Sigma).
Then, 100 μL of the supernatant (FCS) was inoculated on wells formed on solid MRS medium that were seeded by indicator strains, and the wells were incubated for 24 h to 48 h at 37°C.
Samples of 0.1 mL of the K medium that were amended with 50 Mn II1 Mn(II) and submitted to the same conditions of the experiments with the inoculum were mixed with 0.5 mL of 0.04% Leucoberbelin blue I (LBB, Sigma-Aldrich, USA) in 45 mM acetic acid.
Subsequently, we examined the content of lipid and hydrocarbon in log-phase cells (e.g., at the density of ~1.61 g/L in 1× BB medium) that were extracted by methanol/chloroform solution (i.e., the ratio was 2 1 by volume) and hexane, respectively, using gravimetric methodologies (see " Methods").
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com