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Screening was carried out on the SD medium that lacks tryptophan, leucine, histidine and adenine, and is supplemented with 5 mM 3-AT.
For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins.
However, such mutations can be reversed, with the gene regaining its function, and these revertants (his+) are able to grow on medium that lacks histidine.
Single colonies grown on synthetic medium lacking uracil were dispersed in 1 ml synthetic medium that lacks uracil and grown to saturation at 30°C.
Meiosis and sporulation are induced by transferring cells into a medium that lacks nitrogen and contains a nonfermentable carbon source such as acetate.
In S. cerevisiae, autophagy-related pathways can be monitored by transferring cells to starvation medium that lacks nitrogen and amino acids (SD-N) to induce autophagy, for which organelle-specific markers can be followed to monitor specific selective autophagy pathways.
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The growth pattern was similar to what was observed in a medium that lacked Hg2+ amendment (Fig. 1a, d).
It is noteworthy that small quantities of Hg were detected in EPS from medium that lacked HgCl2 amendment probably due to efflux of intracellular Hg in the inoculum, which was also reflected in the EPS as the cells grew.
They clearly couldn't reconcile what they were seeing with their perceived knowledge of film - that it was a flawed medium that lacked the fidelity of digital.
Of six deletion strains tested, all were unable to grow on medium that lacked leucine, implicating this gene as functioning in the leucine biosynthesis pathway of S. pombe.
A natural biofilm-forming photosynthetic consortia was collected from a pond in Columbia, MD and adapted to modified BG-11 medium that lacked citrate as previously described [10].
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Justyna Jupowicz-Kozak
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