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The inventory collectively amounts to the ingredients of an ideally wholesome, happy home — tools for a set of forgotten skills, tastes and virtues I casually refer to as "the household arts" — a medium that includes warm sheets stacked on ironing boards, pies cooling on windowsills, Mason jars full of last summer's gooseberries, and cans of Old Dutch Cleanser under the sink.
Medical writers also write about research discoveries for medical journals, Web sites, newsletters, magazines, newspapers, and any other medium that includes coverage of health and medical issues.
TV is an incredibly collaborative medium" that includes the writer's involvement in camera shots, wardrobe and props.
Tetrahymena grows rapidly in simple rich medium (primarily proteose peptone), with a doubling time of approximately two to three hours [ 45], but can also be grown in simple chemically defined medium that includes a number of amino acids, nucleosides, salts and vitamins [ 31, 46].
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They placed these in a petri dish containing a specialized culture medium that included a component called KSR, which is often used for culturing embryonic stem cells.
Growth of Fas2 KO strains was also analyzed in fatty acid-containing medium that included YPD plus 0.001 to 0.1% (w/v) of myristic acid (14∶0), palmitic acid (16∶0), stearic acid (18∶0), oleic acid (18∶1) or 0.001 to 0.1% (v/v) of Tween 80.
However, in some studies, these cells were grown successfully in the medium that included 2% FCS [ 30].
The cells in the upper chamber were maintained in serum-free medium that included mitomycin-C (10 μg/ml; Sigma-Aldrich), and the lower chamber was filled with culture medium supplemented with 10% fetal bovine serum as the chemoattractant.
After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their respective final concentrations.
The medium was then replaced with medium that included the supplement to induce synchronized growth (0 hr time point) and then treated or not treated with 2.5 μM CdCl2 for 4, 8, 16, or 32 hr.
(A ) RDEB/FB/C7 cells depleted for SLY1 or TANGO1 or control cells were pulsed with S-methionine for 20 min and chased for 2 hr in complete medium that included 5 mg/ml BFA where indicated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com