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They placed these in a petri dish containing a specialized culture medium that included a component called KSR, which is often used for culturing embryonic stem cells.
Growth of Fas2 KO strains was also analyzed in fatty acid-containing medium that included YPD plus 0.001 to 0.1% (w/v) of myristic acid (14∶0), palmitic acid (16∶0), stearic acid (18∶0), oleic acid (18∶1) or 0.001 to 0.1% (v/v) of Tween 80.
However, in some studies, these cells were grown successfully in the medium that included 2% FCS [ 30].
The cells in the upper chamber were maintained in serum-free medium that included mitomycin-C (10 μg/ml; Sigma-Aldrich), and the lower chamber was filled with culture medium supplemented with 10% fetal bovine serum as the chemoattractant.
(A ) RDEB/FB/C7 cells depleted for SLY1 or TANGO1 or control cells were pulsed with S-methionine for 20 min and chased for 2 hr in complete medium that included 5 mg/ml BFA where indicated.
After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their respective final concentrations.
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The inventory collectively amounts to the ingredients of an ideally wholesome, happy home — tools for a set of forgotten skills, tastes and virtues I casually refer to as "the household arts" — a medium that includes warm sheets stacked on ironing boards, pies cooling on windowsills, Mason jars full of last summer's gooseberries, and cans of Old Dutch Cleanser under the sink.
Medical writers also write about research discoveries for medical journals, Web sites, newsletters, magazines, newspapers, and any other medium that includes coverage of health and medical issues.
Meanwhile, movement became just one of an anarchic array of mediums that included song, film, costume, set design and props.
The work of the twenty-six individuand and groups, mostly ranging in age from twenty-five thirty-fiveive, from nineteen countries, is formally conservative, for the most part: lots of painting, and craft mediums that include weaving and ceramics.
The factors affecting porosity was linked to the voltage using Bruggeman's effective medium approximation that included electrical conductivities of the electrolyte and the sample.
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