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A solution of cells, some of which contain the new genome, is mixed with a gel-based culture medium that contains an antibiotic.
In our routine cardiomyocyte maintenance culture medium that contains phenol red, GCaMP6s RhiPSC-CMs still featured a robust optical signal detectable over the fluorescence background (Supplementary Video S2).
It is highly complex medium that contains almost every element in the periodic table and a multifarious plethora of living organisms.
The cells were thereafter fed with a medium that contains the prokaryote Serratia sp. (on which Paramecia feed) and dried Lettuce (Lactuca sativa var. capitata) leaves (on which the prokaryotes feed).
b, GC protection assay schema: bulk GC fraction isolated after subcellular fractionation is a suspension of GC particles enclosing GC-specific molecules (blue) within a medium that contains dilute soluble cytosolic molecules (red).
A defined medium that contains the maximum non-inhibitive concentration of nutrients allows growth of E. coli to a cell density of about 15 g/l dcw (primary source)." Note-Counter-intuitively, the cell density (and the OD) that can be reached when maximal not-inhibitive concentration of nutrient is supplied is lower than cell density when lower concentrations of nutrient are given.
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Human neuroblastoma SH-SY5Y cells were maintained in RPMI 1640 medium that contained 10% FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin4.
Experiments were performed in either calcium-containing or calcium-free medium that contained egtazic acid and after preincubation with the intracellular calcium chelator BAPTA-acetoxymethylester.
Their experiments involved first exposing the mold to mutation-inducing X-rays and then culturing it in a minimal growth medium that contained only the basic nutrients that the wild-type, or nonmutated, strain of mold needed to survive.
Primary astrocytes, prepared from the cerebral cortex of neonatal rats (12 24 h old), were cultured in medium that contained 10% fetal bovine serum in low-glucose Dulbecco's Modified Eagle Medium.
To investigate that question, Ribbeck and her colleagues observed the behavior of Pseudomonas aeruginosa bacteria in a growth medium that contained soluble purified mucins — long proteins with many sugar molecules attached.
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