Human neuroblastoma SH-SY5Y cells were maintained in RPMI 1640 medium that contained 10% FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin4.
Experiments were performed in either calcium-containing or calcium-free medium that contained egtazic acid and after preincubation with the intracellular calcium chelator BAPTA-acetoxymethylester.
To investigate that question, Ribbeck and her colleagues observed the behavior of Pseudomonas aeruginosa bacteria in a growth medium that contained soluble purified mucins — long proteins with many sugar molecules attached.
A wild type strain of Aspergillus niger, denoted as BTL, produced elevated levels of β-galactosidase when grown in a low cost medium that contained wheat bran as the sole carbon and energy source.
Butterfly was planted in a medium that contained 25 mg/kg cadmium (Cd) with application of varying nitrogen concentrations (0 mM, 8 mM, 16 mM, 24 mM and 32 mM) weekly.
Transformed calli were then selected on N6 medium that contained 50 μg l-1 hygromycin.
Solid state fermentation medium that contained peptone and glycerol was found to be a suitable medium for prodigiosin production.
Bacillus subtilis str.168 (Kunst et al. [1997]) was cultured on LB agar medium that contained 1% glucose at 37°C for 18 hrs.
After 24 h of growth, the medium was replaced with the serum-free medium that contained varied concentrations of AuNPs (10 to 100 μM).
Optimal combination of yeasts displaying EG with mutated CBMs was selected from the yeast mixture with comprehensively mutated CBMs in a medium that contained biomass cellulose (newspaper etc).
The basal medium that contained these raw materials were autoclaved at 121 °C for 15 min, and the optimized ions were then added after filter-sterilization.
