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Colour development, resulting from reduction of a redox dye (tetrazolium violet) added to the culture medium (Text S1), was used as a surrogate of metabolic activity, and was measured spectrophotometrically (λ = 600 nm) at 0, 8, 16, 24, 32, 40 and 48 h using a Thermomax microtitre plate reader (Molecular Devices, Wokingham, UK).
Cells were washed by re-suspension of pellets in 7 ml of mineral salts medium (Text S1) and further diluted to the required cell concentration prior to inoculation of 200 µl mineral salts medium, supplemented with the required combination of substrates, with 20 µl of diluted cell suspension.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com