Growth medium temperature was controlled with very small levels of oscillation around the set point by using optimal controller parameters with GPC.
In this study, S. cerevisiae was produced in a batch bioreactor in aerobic conditions and the growth medium temperature was controlled at its optimal value.
The bottle and medium temperature was controlled by putting it in a heated water bath at 35 °C.
In order to control the growth medium temperature, the Generalized Predictive Control (GPC) method was used.
The temperature control medium (40% propylene glycol, Dowcal 20) is, thus, forced to circulate around the wall (Fig. 1b).
The wall of each 1.4-m unit is divided into six interconnected concentric sections with holes in the dividing lamellae on opposite sides of two neighbouring sections (Fig. 1b), and with an inlet for the temperature control medium at the bottom and an outlet at the top section.
In particular, the following changes with respect to the standard industrial cultivation procedures were investigated: the use of a seawater-based medium, none or limited temperature control, nitrogen deprivation when carbohydrates or lipids are the target products.
The study of the reaction between Ca(OH 2 and SO2 has been performed in the medium temperature range (300 450°C) under controlled dehydration conditions.
The reactor concept followed the typical three-stage sequence for numerous organic reactions: non-reactive mixing at low temperature, main conversion with selectivity control at medium temperature, and completion of the reaction at high temperature.
When applied to the task of controlling cell medium temperature, CO2 concentration and relative humidity on VMBs it creates a stable environment that allows cells to multiply on the end of a VMB over a period of 36 h, providing a low-cost (costing less than $2700 to build), customisable alternative to commercial time-lapse microscopy systems.
