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Furthermore, the neurite growth and elongation of PC12 cells are induced by adding the neurotrophin medium suspension produced from SCs-laden conductive films.
DLS was used to measure the hydrodynamic diameters of NPs in Milli-Q water and in medium suspension (100 μg/ml).
Raman spectroscopy gave no indication of differences between raw SWCNT and those deposited from the medium suspension indicating that no debundling of the SWCNT occurred.
The M. bovis BCG culture medium suspension was added to the differentiated cells, centrifuged for 3 min at 800 rpm and subsequently incubated for 4 h.
A medium suspension of passage 2 human chondrocytes was mixed in a 1 1 ratio with liquefied ultrapure agarose (Invitrogen, Carlsbad, CA, USA) and loaded into a stainless steel bioreactor to create four 70 μl constructs of two percent agarose containing 10×10 cells/ml.
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Also upon visual observation of the AuNP suspensions in the different medium suspensions after 24 h of incubation, we made some key observations regarding sedimentation over time.
Due to the limitations of high salt content in both medium suspensions, zeta potential measurements were performed only in Milli-Q water.
Zeta potential measurements were performed to determine the stability of the PBH-capped AuNPs in Milli-Q water and in the different medium suspensions (EMEM/S+ and EMEM/S-).
The stable suspensions of TiO2 ENPs caused immobilization of D. magna, 48-h EC50 value of 13.7 mg L−1 (95% CI, 2.4 mg–79.1 mg L−1); whereas no toxicity was seen in the unstable, highly agglomerated M7 medium suspensions, 48-h EC50 >100 mg L−1.
Medium suspensions with 10∧7 S. mitis were inoculated as first bacterial species in an uncoated 96- or 24-well polystyrene plate, the latter with one coverslip per well, and incubated for 24 h under anaerobic conditions at 37°C.
After 48 h of incubation, cells were scraped and centrifuged in the cell-medium suspension for 5 min at 15 000 × g r.p.m.
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