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Thus, as the only medium supporting growth or at least viability of all three species while simultaneously allowing monolayer/biofilm growth for at least two species, CDM/sucrose was used for all subsequent experiments.
BM cells were analyzed 24 hours after CLP by colony-forming unit assay with medium supporting growth of human cells.
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This medium supported growth of the parental strain BY4743, with all glucose and fructose being consumed within 144 h (data not shown).
Increasing cultivation time up to 72 h resulted in increasing growth yield; P medium was still the best medium supporting the growth of different B. sphaericus strains (about 43 - 300 fold over the other tested media).
Although a chemically-defined medium, supporting high growth rates, is available for L. lactis (Poolman and Konings, 1988), we noted a dramatic decrease in the expression levels of several proteins once the standard complex broth was changed for CDM.
The environment consisted of a serial transfer regime in which the populations were diluted each day into a glucose medium, supporting exponential growth for a limited time.
We agree that ensuring the flow of credit to viable SMEs [small and medium enterprises] is essential for supporting growth and should be a core priority for a new government, and we will work together to develop effective proposals to do so.
Similar observations were made for CCT knockout heterokaryon progeny that were isolated into MEPP medium that supports growth of cells lacking either a functional oral apparatus [25] or cilia [26], [27].
A novel liquid culture medium that supported growth of Bartonella spp. also used fresh, defibrinated sheep blood as a growth supplement (10 ).
The inoculum was standardized to ∼1×10 CFU mL−1 and diluted 1 100 in Middlebrook 7H9 broth (Middlebrook ADC enrichment, a dehydrated culture medium which supports growth of mycobacterial species, available from Becton Dickinson, cat. # 211887), to produce the final inoculum of H37Rv strain (ATCC25618).
From this perspective, the American Jobs Act proposed by President Obama can play an important role in supporting growth and employment, if it is embedded in an appropriate medium-term framework to bring down the public debt.
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