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Cell culture medium, medium supplements, and compounds were obtained from Sigma-Aldrich (Sweden) or Life Technologies Swedenn) unless otherwise stated.
Cell culture conditions are laboratory specific and may differ with respect to cell lines, medium supplements, and incubation temperatures.
It is a statistical design providing an efficient way of screening a large number of medium supplements and identifying the important factors.
We established the SVR model for prediction, since we tend to get the growth rate of CHO with 7 medium supplements and to recognize the most important components of them.
Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B12, inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640.
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Murine macrophages (RAW 264.7 and 23ScCr) and human kidney epithelial cells HEK293 were grown in Dulbecco's Eagle medium supplemented and incubated as noted above.
Murine macrophages (RAW 264.7, 23ScCr) and human kidney epithelial cells HEK293 were grown in Dulbecco's Eagle medium supplemented and incubated as mentioned above.
On day 5 of differentiation, the culture medium was replaced with RPMI-B27 medium without supplements, and medium was exchanged every 3 days thereafter.
This review analyzes the advantages and limits of using human platelet materials as medium supplements for MSC isolation and expansion.
We will continue testing additional growth factors and medium supplements in future studies.
The water potentials of media (MS medium supplemented with and without PEG) were: −0.3 MPa for control; −0.45 MPa for 2% PEG and −0.6 MPa for 4% PEG.
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