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Further medium supplementation with FGF-2 stimulated early cell proliferation (>3 fold higher total DNA content per explant at day 10).
Culture medium supplementation with non-animal derived components including recombinant proteins and lipids of synthetic or non-animal-derived origin resulted in improved HEK 293 cell growth and VLP production.
The aims of this study were (1) to determine the effect of in vitro maturation (IVM) medium supplementation with MEM vitamins on in vitro embryo development and sensitivity to vitrification of Day 6 blastocysts and (2) to evaluate whether the addition of forskolin to in vitro culture (IVC) medium enhances blastocyst survival following Super Open Pulled Straw (SOPS) vitrification.
Effect of medium supplementation with yeast extract and peptone on ethanol production was studied.
Chondroitin concentration remained unchanged following medium supplementation with 0.5 mM GalNAc, whereas it was slightly lower in the presence of phosphatidylcholine.
Chondrogenesis induced by scaffold-mediated gene delivery was as effective as traditional differentiation protocols involving medium supplementation with TGF-β3, as assessed by gene expression, biochemical, and biomechanical analyses.
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The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone production were examined.
The fermentation of jatropha seed cake with medium supplementation (maltose and peptone 2%, 4040 % moisture, and 25 °C produced maximum amount of proteases (3366 U/g) and lipases (1288 U/g) and removed jatropha seed cake anti-nutrients (Veerabhadrappa et al. 2014).
The cells were differentiated into macrophages by seeding the cells, at a density of 5 × 104 cells per circular cover slip (diameter = 13 mm) (Glasscyto, Brazil), and placing them into each plate well (24-well plate), with resuspension in U937 medium and supplementation with 10 nM PMA for 3 days at 37°C under 5% CO2 atmosphere.
Enzymes 1, 3 8, and 11 were expressed in ER2566 E. coli cells (New England Biolabs) and grown in Terrific broth medium with supplementation with riboflavin, niacinamide, and pyridoxine (20 mg/L each).
Furthermore, several microalgae that are grown in pure culture with mineral medium require supplementation with the vitamins cobalamin and/or thiamine (Carvalho et al. 2006; Croft et al. 2005; Droop 2007).
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