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The transport of bovine serum albumin (BSA) and of cell culture medium supplement with 10% fetal bovine serum (FBS) through the produced PLLA fiber is high (permeance 1963 L/(m2 h bar)) with low protein retention.
For naïve state conversion, cells cultured in standard hESC medium on MEFs were dissociated to single cells using 0.05% trypsin/EDTA solution (Invitrogen), replated on MEFs, and cultured overnight in standard hESC medium supplement with 10 μmol/L Rho Kinase (ROCK -inhibitor Y-27632 (Calbiochem).
PAG cells were grown in Ham's F10 medium supplement with 15% FBS, Pen/Strep and 1× MITO + (BD Biosciences).
Monocytes with >95% purity were suspended at 1×106 cells/mL in RPMI 1640 medium supplement with 10% FBS (FBS contained <5 pg/100 mL LPS).
Strikingly, the ldlD-LG cells produced the least amount functional glycans when grown in the medium supplement with both Gal and GalNAc, despite the glycosylation pathways were restored and evidenced by the β-DG immuno-blotting (Fig. 5).
After homogenization, the material was tenfold serially diluted in Schneider medium supplement with 10% foetal bovine serum.
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Subsequently, cultures serially diluted and spread on CDMM medium supplemented with FOA and uracil.
Bacterial cells were incubated overnight at 37 °C in LB medium supplemented with ampicillin and kanamycin.
BioID transfected cells were cultured in medium supplemented with 50 μM biotin.
This strain was cultivated in medium supplemented with ampicillin and gentamycin.
KC were resuspended in Advanced DMEM medium supplemented with Thawing/Plating Supplement Pack (Invitrogen) without Dexamethasone.
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