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After 1 h, the BWW medium supernatant layer was removed and centrifuged at 400 g for 5 min to collect the spermatozoa.
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Four distinct layers (supernatant, layer including bacteria and viruses, Nycodenz, dust pellet) were clearly visible when the sample was observed against a light source.
The mycelium-free medium supernatant was applied to 150 g of pre-equilibrated Diaion HP-20 resin packed in a column (40 × 220 mm).
Medium supernatant was assayed for Gluc activity.
The supernatant layer was separated and evaporated at 40°C.
24 hours after incubation, we collected the medium supernatants or the proteins for detection.
Cytoplasmic extracts and corresponding medium supernatants were analysed for amino acid composition by gas chromatography.
Sattler's medium vessel layer.
A layer of the choroid, the medium sized layer, is commonly heavily pigmented.
The idea is to have a lightly packed bottom layer, a medium middle layer, and a tight top layer.
The supernatant toluene layer was collected and dried over Na2SO4.
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