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The membranes were medium stripped with stripping buffer (1.5% Gly, 0.1% SDS, 1% Tween20, pH 2.2), PBS and Tris buffered saline containing 0.1% Tween 20 and incubated with anti-GST antibodies.
Furthermore, MSC-conditioned medium stripped of IL-6 induced the same growth rates observed in the Skov-3 tumor cells alone (Figure 5B).
To verify that each GF had been removed from the conditioned medium, an aliquot of conditioned medium stripped of GF via immunoprecipitation was tested for the respective GF concentration by ELISA, and in all cases, GF concentrations were >14-fold less as determined by ELISA.
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After medium stripping the membrane the blot was reprobed with anti-GFP antibodies.
The membrane was reprobed with anti-GFP antibodies after medium stripping.
Vancomycin, cefoxitin, cefotaxime, ceftazidime, piperacillin tazobactam and meropenem Etest strips were then applied to the inoculated medium strips selected to aid detection of resistant Gram-negative pathogens.
For all experiments using PRL-HeLa, cells were grown a minimum of two days in hormone-free medium containing stripped and dialyzed fetal bovine serum.
Furthermore, MCF7 cells kept for many years under oestrogen-depleted conditions using medium containing stripped foetal bovine serum produce rapid apoptosis via an intrinsic mechanism directed at the mitochondrion [ 40, 41].
Perception is a rarity in video gaming, a highly visual medium – it strips its central character, Cassie, of her sight.
Transient transfection experiments were performed in RPMI-1640 medium supplemented with 10% charcoal/dextran-treated FBS (stripped medium).
After 6 h the medium was replaced with complete medium containing 10% stripped fetal bovine serum, 2 mM glutamine, 50 U/ml penicillin, 50 µg/ml streptomycin and the cells were incubated overnight.
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