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Normal breast tissue showed no staining for IFITM1 or PLSCR1 (SI score = 0); primary tumors showed medium staining for IFITM1 and PLSCR1 which correlated with low expression (SI score of ≤3); and AI-resistant (recurrence) tumors showed very strong staining for IFITM1 and PLSCR1 which correlated with high expression of both proteins (SI score of ≥6).
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Cells expanded in MesenCult medium stained positive for Sca-1, CD29, CD44, CD105, CD106, and, in part, CD146 and CD14.
Pellets formed in chondrogenic medium stained positive for sulphated-GAGs, consistent with SF and ICBM controls and indicative of progression toward the chondrogenic lineage.
Let set for two days for medium stain or 4 days for darker stain.
Ten percent of invasive glioma cells showed strong staining for CTGF, 70% exhibited medium and 20% weak immuno-positivity while 80% of glioma cells in core samples exhibited weak staining and 20% exhibited medium staining.
Slides were counter stained with haematoxylin and mounted with Apathy's mounting medium Immunohistochemical staining for VEGF was carried out using the streptavidin-biotin-alkaline phosphatase (ABC) method, as previously described (Zhang et al, 1998).
Stain the board with a light to medium stain tone.
mDCs obtained from monocytes generated in the presence of rtv, apv or regular medium showed comparable staining for NK2DG-Fc.
Any suspected growth on LJ medium was confirmed by ZN staining for AFB, and the positive growths were sub-cultured on fresh LJ media for further identification and sensitivity.
Monolayer cultures of MSCs responded equivalently to osteogenic or adipogenic medium in terms of strong staining for ALP, mineralisation with Alizarin Red, or staining with Oil Red O for lipid droplets, while only weak staining was observed in the respective control cultures.
Cells treated with the control medium for 72 h showed prominent staining for HA.
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