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Two glass columns were packed with soil obtained from a trichloroethene-contaminated site at Cape Canaveral, Florida, and were fed a minimal medium spiked with PCE (7 μM) for 391 days.
The seeds, they found, germinated in growth medium spiked with TNT or nitroglycerin, an environment that kills normal seeds.
Table 3 presents the results acquired from DNA tests carried out with the hybridisation medium spiked with different strains of V. cholerae DNAs and other bacterial species at a concentration within the calibration range of the DNA biosensor.
For NIR measurements of the heat-shocked cells, heated bacteria were spiked into fresh medium and immediately transferred into one test chamber of the cassette; the adjacent control chamber was filled with the same medium spiked with PBS.
Then 100 µl medium was removed and replaced with 100 ul CM (sample) or 100 µl unconditioned medium spiked with varying concentrations of TGF-β1 to establish a standard curve.
Cells were treated with 1 ml of medium spiked with appropriate concentrations of EL102, docetaxel or both.
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To quantify eicosanoids excreted in the medium, Eppendorf tubes containing 0.6 mL of F12K medium were spiked with lipid standards, together with internal standards for [C20]-15-oxo-ETE [C20]-15-oxo-ETE [C20]-15-oxo-ETE [C20]-15-oxo-ETE-15-keto-PGE2-PFB (1 ng each).
Non-supplemented DW medium was spiked with several N sources (Table 2): no supplement as a reference, (NH4 2SO4 as the usual N supplement, (NH4 2CO3 as an alternative ammonium resource, and urea as another N compound.
When medium was spiked with Cd, with an increase in Cd concentration, there was an increase in GST activity in both roots and shoots of transgenic tobacco plants up to 100 µM Cd (Figure 4A, B).
Usually, the medium was spiked with benzene (120 μM) or benzoate (700 μM) as carbon and energy sources, respectively.
For HPLC analysis, samples of medium were sometimes spiked with 10 μg mL−1 11β-OH-T as internal standard to generate controls and extracted with diethyl ether as described above.
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