As Koestenbaum writes, the dominant strategy of his Pop was to combine "lurid subject" and "cool presentation", and to translate images from one medium or sphere into another in order to "embalm" them - though this embalming could also infuse his images with a psychic charge, an unexpected punctum, as Roland Barthes might say.
Last, we stress that subroutines for calculating the fluorescence signal emitted by a localized fluorescent inclusion placed inside a two-layer medium, a sphere, a parallelepiped, and a cylinder, can be obtained by using the software package provided in [ 21] and by implementing for these geometries a similar approach to that used for the infinite medium and the slab.
When cultured in a serum-replacement medium, the spheres maintained their stem markers for months.
After 12 days of culture in growth medium, hSKP spheres were trypsinized and pipetted into single cells.
The mucosa tissue was described as a suspension of medium collagen spheres (r = 200 nm) at 12% volume fraction in physiological liquid (MFP = 53.7 µm, m = 1.46/1.38, h 1 = 0.5 mm).
The 9 virtual objects included a small, medium, and large sphere, as well as a sphere of each size where the circumference transitioned to a straight line at either the 135° or 45° location.
To passage the spheres, medium was aspirated off and spheres were harvested with the TransferMan NK 2 micromanipulator (Eppendorf).
The use of the threshold-based and the background-adapted methods in the 68Ga-PET images resulted in recovery coefficients close to 1 for medium-sized spheres (17 37-mm 17 37-mm).
Two days after plating, supernatant was collected and replated in a defined serum-free tumor sphere medium formed by Ham's F-12/Dulbecco's modified Eagle's medium (1∶1) supplemented with B27 (Invitrogen, Carlsbad, CA), 20 ng/ml epidermal growth factor (EGF, Peprotech, EC) and 20 ng/ml fibroblast growth factor (FGF, Peprotech, EC).
After 48 h from transfection, cultures were grown in sphere medium and allowed to form spheroids.
