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A total of 5×104 cells in 1 ml of appropriate medium (specific for each cell line, see above) were seeded into each of a 12 well plate and treated at different concentrations of drugs.
A medium specific for endothelial cell culture enabled ESP cells to proliferate and differentiate into various types of endometrial cells, including glandular epithelial, stromal and endothelial cells in vitro, whereas in the same medium, EMP cells differentiated only into stromal cells.
The hADSCs were purified by sequential passage of SVF cells in a culture medium specific for the mesenchymal cell lineage.
The metabolic diversity of this group is indicated by the lack of a culture medium specific for sphingomonads, leaving the molecular approaches more appropriate for their study.
The clonal Leishmania donovani cell line LdBOB (derived from MHOM/SD/62/1S-CL2D MHOM/SD/62/1S-CL2Der promastigotes or axenic amastigotes in medium specific for each developmental stage (17).
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This accounts for the specific surface area of the porous medium (specific pore volume) or the total area exposed within the pore space per unit of grain volume.
The cells were starved for two days, then left unstimulated, or simulated with 50 ng/ml PDGF-BB (Sigma-Aldrich, Saint Louis, MO) in six well plates in serum-free medium for specific time points.
Cells were treated with chemotherapy at 15%to30%0% cell density for 1 3 days, rinsed with PBS and allowed to recover at indicated times in complete growth medium for specific assays.
What's most interesting, though, is the medium-specific reference that Bader slips in: for a few years, scientists have been studying the structure of sea urchins' spines in a quest to produce erosion-resistant concrete.
An operative cohort study of women aged 18 44 years found biological-medium-specific effects of POPs on endometriosis: for example, PCB 151 in omentum fat was significantly associated with increased risk of endometriosis, but not PCB 151 in serum [ 50].
However, the effects of high and medium-tech are not specific for the western or eastern parts of the country.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com