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After mounting with Dako fluorescent mounting medium, slides were observed by fluorescence microscopy.
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A few medium size slides are seen mainly in the middle portions of the valley.
The mitotic chromosomes of the generative cells are allowed to proceed through mitosis in the culture medium and slides are prepared for metaphase figures.
Cells were fixed with 90% acetone (10 min) and then were treated with human CD31 (1/100, Invitrogen, 45 min) and Alexa 488 + Hoechst 33258 (30 min) and covered with mounting medium on slides.
Up to this point they have been the beneficiaries of television executives' aggressive search for programming that will halt the medium's slide in popularity with younger audiences, even if, as in the case of Mr. Lehre, they're not sure what they're getting.
Distilled water as a medium for slide mounting.
Intermittent small and medium scaled sliding was reported in the aftermath of the events.
Other than serving as transport medium, dip slide testing has no added to diagnose or exclude significant bacteriuria.
A coverslip was placed on the slide with mounting medium, and the slide was examined under an epifluorescent microscope.
Parental FaDu, SCC25, CAL27 and radioresistant FaDu-IRR, SCC25-IRR and CAL27-IRR cells were plated at a concentration 1 × 10 cells in 0.5 ml medium onto chambered slides (8-chamber polysterene vessel tissue culture-treated glass slide, Falcon, Becton Dickinson Labware Europe, Le Pont De Claix, France) and incubated in CO2 humidified atmosphere at 37 °C.
The cells were washed twice in PBS and then mounted by inverting onto mounting medium on glass slides.
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