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As a consequence, clustering of small to medium sized libraries with up to 1 million compounds is regularly performed using deterministic techniques whereas libraries comprising millions of compounds are mostly clustered using heuristics like k-means [3].
"It's the big and medium size libraries that have really started to get into this seriously," says Sarah Holmes of Three Rivers Freenet, a community and nonprofit access Internet network in Pittsburgh, Pa.
The medium sized insert library was constructed using a pHOSII cloning vector.
The antigen specific entity was then spiked into an irrelevant background at a 1∶107 level giving a known diversity of 107, corresponding to a medium sized combinatorial library.
The two medium-size libraries (5 kbp and 8 kbp) were prepared using the GS FLX Titanium Kit (Roche) following standard protocols.
During the initial random-sequencing phase, 8-fold sequence coverage was achieved with the small- and medium-size libraries sequenced to yield 5-fold and 3-fold coverage, respectively.
I have medium sized sheep.
Band-winged is a medium sized nightjar.
A medium sized bronze and green hummingbird.
In medium sized bowl, blend syrup and eggs.
A medium sized lobby group hit right back.
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