Sentence examples for medium selection for from inspiring English sources

Along with the liquid medium (FNL) selection we also tried the solid (D20) medium selection for soybean somatic embryos for LT and AEC.

After 5 days, the cells were split 1 : 3, and 1 mg/mL G418 was added to the medium for selection for 10 days; the medium was replaced with fresh medium every 2 to 3 days.

The various deletion strains used in this study were cultured in non-inducing medium with selection for plasmids supplemented with 0.4 g/l G418 (Geneticin) or inducing media with selection for plasmids supplemented with 0.4 g/l G418.

Cells were grown at permissive temperature in YPD; for maintaining plasmids, in minimal medium; for selection of kanMX4 carrying strains with 100 μg/ml G418; or for selection of ura-cells with 5-fluoroorotic acid (5-FOA) (Boeke et al, 1987) and 1 M sorbitol.

SCRaMbLE was then induced in a growing culture of this strain for 4 h and cells plated onto SDO URA− agar medium to maintain selection for the violacein plasmid.

It is separable from the solids by filtration or centrifugation and is characteristically rich in xylose but low in glucose, a feature which may make PSGHL a useful enrichment medium to force selection for improved xylose utilization in hydrolyzates, a failing point for many types of native and engineered yeast tried in the past.

The transformants were spread on SPASC medium for selection, and several transformants were selected, based on colony size.

After transfection according to the methods mentioned above, 20 µg/ml puromycin was added into medium for selection.

Forty-eight hours after the second infection, 350  μg ml−1 of hygromycin was added to the culture medium for selection of infected cells.

The pUG35 vector has -URA (uracyl) promoter, so the cells are growing on the uracyl-depleted medium (for selection); the protein expression is unregulated because it is immediate expressed upon transfection.

S. pneumoniae cells were grown in AGCH medium [40] supplemented with 0.3% sucrose and 0.2% yeast extract (complete AGCH) medium, with or without selection for resistance to chloramphenicol (CmR, 2 µg. ml−1).