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After the treatment, the cells were screened by medium selection and restriction enzymes.
Primary cultivation method was improved from the procedures of animal selection, lung tissue perfusion, tissue piece pasting, culture medium selection and so on.
Generally, there are three main steps involved in green synthesis method, i.e., reaction medium selection, biological reducing agent selection, and selection of non-carcinogenic substances for stability of nanoparticles (El-Shishtawy et al. 2011).
Potential approaches for equipment design and maintenance, operational procedures, validation/verification testing, medium selection, culture purity/sterility analysis, and contamination investigation are presented, and those approaches implemented are identified.
The most sensitive factor on transmittance is obtained by the method of (NFDTD) according to the orthogonal numerical analysis, which may be helpful to the nonlinear Kerr medium selection in the photonic crystal waveguide design.
In this study, we evaluated the interactive effects of plant species and growth medium selection on rainwater capture, nitrogen and phosphorus content of runoff, and production of edible biomass.
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The transformants were spread on SPASC medium for selection, and several transformants were selected, based on colony size.
Bacteria from fresh selective agar plates were inoculated into 2 ml LB medium with selection antibiotics and incubated over-night at 26°C with shaking.
Development of economical medium requires selection of carbon, nitrogen, phosphorous, potassium and trace element sources.
Cells were incubated in the medium (containing selection drug) 24 h after transfection.
After transfection according to the methods mentioned above, 20 µg/ml puromycin was added into medium for selection.
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