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After another very brief vortexing, ca. 0.2 mL was removed for the evaluations of germination and growth on selective medium (see below).
This process was repeated three times to wash the cells, which were then resuspended in 3 mL LB or 3 mL pre-treated LB medium (see below).
One gram of the straw/bran blend was mixed with 5 ml minimal medium (see below; excluding a C-source) and autoclaved (121°C for 15 min) in a 100 ml Erlenmeyer flask.
After cultivation for 2 days at 30°C on BHI agar plates containing 25 μg mL−1 kanamycin, the transformants were selected for a strain with a single crossover of the ΔpknG genotype, which was then cultivated in 5 mL of BHI liquid medium at 30°C overnight and diluted 1 10,000 with MM medium (see below) containing 10% sucrose.
These cells expressed glycolytic genes from inducible promoters (Psapc-I and Pxyl) or carried the ΔpykA mutation and were grown in a gluconeogenic medium (see below).
In the case of EB formation using mouse testicular or ovarian cell-conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned medium.
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1015E; see below, sect.
(See below, Section 5.3).
(See below, sect. 3).
In creating their campaigns, students should be encouraged to choose the medium (see list below) that would most effectively reach their target audience.
Sporozoites are then recovered in the pellet and resuspended in complete culture medium (see composition below).
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